Staining:Article Title: Enteric botulinum neurotoxins facilitate infection by Salmonella and Shigella
Article Snippet: Magnetic Dynabeads M-280 streptavidin were from Invitrogen–Life Technologies (Life Technologies AS, Oslo, Norway). .. For IF staining, the following antibodies were used: anti-VAChT (1:500; catalog no. 139 105, primary antibody, Synaptic System), anti-TH (1:1000; catalog no. ab76442, primary antibody, Abcam), anti-Substance P (SP) (1:500; catalog no. PA5-106886, primary antibody, Thermo Fisher Scientific), anti-VIP (1:200; catalog no. MAB6079-SP, primary antibody, R&D System), anti-WGA fluorescein–conjugated antibody specific for mucin (1:300; catalog no. FL-1021, Vector Laboratories), anti-GFP (1:200; catalog no. Ab13970, Abcam), anti–guinea pig Alexa 555–conjugated (1:200; catalog no. A-21345, secondary antibody, Thermo Fisher Scientific), anti-rabbit Alexa 488–conjugated (1:200; catalog no. A-11090, secondary antibody, Life Technologies), anti-mouse Alexa 555–conjugated (1:200; catalog no. A-21422, secondary antibody, Life Technologies), and anti-chicken Alexa 488–conjugated (1:200; catalog no. A-11039, secondary antibody, Thermo Fisher Scientific), as well as 4′,6-diamidino-2-phenylindole (DAPI; 1:5000; catalog no. D3571, Invitrogen) and OCT (catalog no. 1416912.30, Vetrotecnica). α-Bungarotoxin Alexa 488–conjugated (1:200; catalog no. B35451 , Life Technologies) was used to stain the nicotinic ACh receptor. .. Supernatant from cultures of C. botulinum American Type Culture Collection (ATCC) 19397 and 27765 (sBoNT/A and sBoNT/B, respectively) was prepared by centrifugation and ultrafiltration across 0.22-μm filters.
Article Title: Enteric botulinum neurotoxins facilitate infection by Salmonella and Shigella .
Article Snippet: Magnetic Dynabeads M- 280 streptavidin were from Invitrogen–Life Technologies (Life Technologies AS, Oslo, Norway). .. For IF staining, the following antibodies were used: anti- VAChT (1:500; catalog no. 139 105, primary antibody, Synaptic System), anti- TH (1:1000; catalog no. ab76442, primary antibody, Abcam), anti- Substance P (SP) (1:500; catalog no. PA5- 106886, primary antibody, Thermo Fisher Scientific), anti- VIP (1:200; catalog no. MAB6079- SP, primary antibody, R&D System), anti- WGA fluorescein– conjugated antibody specific for mucin (1:300; catalog no. FL- 1021, Vector Laboratories), anti- GFP (1:200; catalog no. Ab13970, Abcam), anti–guinea pig Alexa 555–conjugated (1:200; catalog no. A- 21345, secondary antibody, Thermo Fisher Scientific), anti- rabbit Alexa 488– conjugated (1:200; catalog no. A- 11090, secondary antibody, Life Technologies), anti- mouse Alexa 555–conjugated (1:200; catalog no. A- 21422, secondary antibody, Life Technologies), and anti- chicken Alexa 488–conjugated (1:200; catalog no. A- 11039, secondary antibody, Thermo Fisher Scientific), as well as 4′,6-diamidino-2-phenylindole (DAPI; 1:5000; catalog no. D3571, Invitrogen) and OCT (catalog no. 1416912.30, Vetrotecnica). α- Bungarotoxin Alexa 488–conjugated (1:200; catalog no. B35451, Life Technologies) was used to stain the nicotinic ACh receptor. .. Supernatant from cultures of C. botulinum American Type Culture Collection (ATCC) 19397 and 27765 (sBoNT/A and sBoNT/B, respectively) was prepared by centrifugation and ultrafiltration across 0.22- μm filters.
Whole Genome Amplification:Article Title: Enteric botulinum neurotoxins facilitate infection by Salmonella and Shigella .
Article Snippet: Magnetic Dynabeads M- 280 streptavidin were from Invitrogen–Life Technologies (Life Technologies AS, Oslo, Norway). .. For IF staining, the following antibodies were used: anti- VAChT (1:500; catalog no. 139 105, primary antibody, Synaptic System), anti- TH (1:1000; catalog no. ab76442, primary antibody, Abcam), anti- Substance P (SP) (1:500; catalog no. PA5- 106886, primary antibody, Thermo Fisher Scientific), anti- VIP (1:200; catalog no. MAB6079- SP, primary antibody, R&D System), anti- WGA fluorescein– conjugated antibody specific for mucin (1:300; catalog no. FL- 1021, Vector Laboratories), anti- GFP (1:200; catalog no. Ab13970, Abcam), anti–guinea pig Alexa 555–conjugated (1:200; catalog no. A- 21345, secondary antibody, Thermo Fisher Scientific), anti- rabbit Alexa 488– conjugated (1:200; catalog no. A- 11090, secondary antibody, Life Technologies), anti- mouse Alexa 555–conjugated (1:200; catalog no. A- 21422, secondary antibody, Life Technologies), and anti- chicken Alexa 488–conjugated (1:200; catalog no. A- 11039, secondary antibody, Thermo Fisher Scientific), as well as 4′,6-diamidino-2-phenylindole (DAPI; 1:5000; catalog no. D3571, Invitrogen) and OCT (catalog no. 1416912.30, Vetrotecnica). α- Bungarotoxin Alexa 488–conjugated (1:200; catalog no. B35451, Life Technologies) was used to stain the nicotinic ACh receptor. .. Supernatant from cultures of C. botulinum American Type Culture Collection (ATCC) 19397 and 27765 (sBoNT/A and sBoNT/B, respectively) was prepared by centrifugation and ultrafiltration across 0.22- μm filters.
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